diff --git a/analysis/16S/16S_Analysis_pipeline_v4-3.Rmd b/analysis/16S/16S_Analysis_pipeline_v4-3.Rmd
index 02f27bebb6d2b9e1f99a9bd791978d5ea3c99e09..ab70a85cace8c70120839fc174d99bbaf5fdd29e 100644
--- a/analysis/16S/16S_Analysis_pipeline_v4-3.Rmd
+++ b/analysis/16S/16S_Analysis_pipeline_v4-3.Rmd
@@ -843,7 +843,7 @@ set$ps.topnTax <- cuphyr::abundant_tax_physeq(ps.trans, lvl = set$taxlvl,
 set$my_scale_fill <- my_scale_fill
 
 
-set$topntax_tbl <- psmelt(set$ps.topnTax) %>% 
+set$topntax_tb <- psmelt(set$ps.topnTax) %>% 
                     as_tibble() %>%
                     left_join(set$alpha_div_ps_trans, by = "Sample") %>% 
                     select(Genus, Alias, ndvi, Abundance, Shannon) %>%
@@ -853,6 +853,17 @@ set$topntax_tbl <- psmelt(set$ps.topnTax) %>%
                     arrange(ndvi) %>%
                     mutate(ndvi_rank = c(1:length(ndvi))) 
                     
+set$other <- set$topntax_tb %>% 
+  group_by(Alias) %>% 
+  summarise(sumabu = sum(Abundance)) %>% 
+  mutate(Genus = 'Other', Abundance = 1-sumabu) %>% 
+  select(-sumabu)
+
+set$topntax_tbl <- bind_rows(set$topntax_tb, set$other) %>% 
+  mutate(Genus = factor(Genus, levels = c(
+    sort(unique(set$topntax_tb$Genus)),
+    "Other")
+    ))
 
 plots$topn_tax_custom <- ggplot(set$topntax_tbl, aes(x = fct_reorder(Alias, ndvi),
                            y = Abundance,
diff --git a/analysis/16S/16S_DADA2_results_260821/analysis_output/Controls_13-03-25_160718.pdf b/analysis/16S/16S_DADA2_results_260821/analysis_output/Controls_13-03-25_160718.pdf
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